The inhibition of mitochondrial dicarboxylate transport by inorganic phosphate, some phosphate esters and some phosphonate compounds
نویسندگان
چکیده
منابع مشابه
The inhibition of mitochondrial dicarboxylate transport by inorganic phosphate, some phosphate esters and some phosphonate compounds.
1. P(i) competitively inhibited succinate oxidation by intact uncoupled mitochondria in the presence of sufficient N-ethylmaleimide to block the phosphate carrier, with a K(i) of 2.5mm. 2. Of a large number of phosphate esters and phosphonate compounds, phenyl phosphate and phenylphosphonate were found to inhibit competitively uncoupled succinate oxidation by intact but not broken mitochondria....
متن کاملThe transport of inorganic phosphate by the mitochondrial dicarboxylate carrier.
1. N-Ethylmaleimide inhibited the influx and efflux of P(i) in rat liver mitochondria. 2. The efflux was stimulated by either succinate or malate in the presence of N-ethylmaleimide, and this stimulation was reversed by 2-n-butylmalonate. 2-Oxoglutarate and citrate, even in the presence of low concentrations of malate, were relatively ineffective in stimulating efflux of P(i) under these condit...
متن کاملMitochondrial Phosphate Transport
The mitochondrial phosphate transport protein (PTP) has been purified in a reconstitutively active form from Saccharomyces cerevisiae and Candidaparapsilosis. ADP/ATP carriers that copurify have been identified. The PTP from S. cerevisiae migrates as a single band (35 kDa) in sodium dodecyl sulfate gels with the same mobility as the N-ethylmaleimide-alkylated beef heart PTP. It does not cross-r...
متن کاملThe transport of radioactive inorganic phosphate by the pulmonary circulation.
IN the measurement of cardiac output by the flow-dilution procedure, the injected substance must pass through the pulmonary circulation without loss from blood. The ideal substance for this purpose would, on the other hand, completely disappear in one passage through the systemic circulation, and thus obviate the possibility of errors due to recirculation. The use of such substances has been di...
متن کاملThe determination of inorganic phosphate in the presence of labile phosphate esters.
Labile phosphate esters such as phosphocreatine, acetyl phosphate, and ribose-l-phosphate (1) have assumed great biological importance. These esters are so unstable that they are split with great rapidity by the reagents commonly used for inorganic phosphate determination. Therefore, the usual inorganic phosphate measurements in tissue extracts, etc., actually represent the sum of the inorganic...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1974
ISSN: 0306-3283
DOI: 10.1042/bj1380171